Overall Categorization
Attributes of FASTQ
Alphabet Frequency & GC content
mushroom
Attributes of FASTQ
Name Value InputFile mushroom.fastq #Read 1,000,000 #Base 236,396,829 AvgReadLen 236.40 MinReadLen 25 MaxReadLen 251
Alphabet Frequency & GC content
Name Count Freq% A 64,835,436 27.43% C 53,464,399 22.62% G 53,028,543 22.43% T 65,034,454 27.51% N 33,997 0.01% GC% - 45.05%
Distribution of Bases' Quality Values
Name AreaFreq Q30 & above 89.4% Q20-Q29 4.3% Q15-Q19 3.0% < Q15 3.3%
Distribution of Reads' MinimalQ Values
Name AreaFreq % of reads whose bases are all Q20 & above 21.9% % of reads whose bases are all Q15 & above 23.9% % of reads whose bases are all Q10 & above 98.8%
Coverage of Reads with Sufficient High-Quality Bases
Name Coverage of reads Remark % of High-quality reads
Coverage of reads that 100% of their bases with Q20 & above 21.9% %HighQ(20) >= 100%
(i.e., MinimaQ>=20) Coverage of reads that >= 95% of their bases with Q20 & above 65.8% %HighQ(20) >= 95% Coverage of reads that >= 90% of their bases with Q20 & above 77.8% %HighQ(20) >= 90% Coverage of reads that >= 90% of their bases with Q15 & above 90.4% %HighQ(15) >= 90% % of Poor-quality reads
Coverage of reads that > 10% of their bases with Q14 & less 9.6% 1 - {%HighQ(15) >= 90%}
Attributes of FASTQ
| Name | Value |
|---|---|
| InputFile | mushroom.fastq |
| #Read | 1,000,000 |
| #Base | 236,396,829 |
| AvgReadLen | 236.40 |
| MinReadLen | 25 |
| MaxReadLen | 251 |
Alphabet Frequency & GC content
| Name | Count | Freq% |
|---|---|---|
| A | 64,835,436 | 27.43% |
| C | 53,464,399 | 22.62% |
| G | 53,028,543 | 22.43% |
| T | 65,034,454 | 27.51% |
| N | 33,997 | 0.01% |
| GC% | - | 45.05% |
Distribution of Bases' Quality Values
| Name | AreaFreq |
|---|---|
| Q30 & above | 89.4% |
| Q20-Q29 | 4.3% |
| Q15-Q19 | 3.0% |
| < Q15 | 3.3% |
Distribution of Reads' MinimalQ Values
| Name | AreaFreq |
|---|---|
| % of reads whose bases are all Q20 & above | 21.9% |
| % of reads whose bases are all Q15 & above | 23.9% |
| % of reads whose bases are all Q10 & above | 98.8% |
Coverage of Reads with Sufficient High-Quality Bases
| Name | Coverage of reads | Remark |
|---|---|---|
| % of High-quality reads Coverage of reads that 100% of their bases with Q20 & above | 21.9% | %HighQ(20) >= 100% (i.e., MinimaQ>=20) |
| Coverage of reads that >= 95% of their bases with Q20 & above | 65.8% | %HighQ(20) >= 95% |
| Coverage of reads that >= 90% of their bases with Q20 & above | 77.8% | %HighQ(20) >= 90% |
| Coverage of reads that >= 90% of their bases with Q15 & above | 90.4% | %HighQ(15) >= 90% |
| % of Poor-quality reads Coverage of reads that > 10% of their bases with Q14 & less | 9.6% | 1 - {%HighQ(15) >= 90%} |
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